Junior High School integrated science teachers’ knowledge is a variable that determines the effectiveness of the Junior High School integrated science teachers. Junior High School integrated science teachers’ effectiveness is not very often determined by output of work, but by Junior High School integrated science teachers’ qualification. Ghana Education Service uses West African Senior Secondary Certificate (WASSCE) or Senior Secondary School Certificate Examination (SSSCE) as the minimum qualification needed for employment as non-professional Junior High School integrated science teachers. This arrangement raises an issue relating to quality. Do the WASSCE/SSSCE certificate holders possess Junior High School integrated science knowledge similar to Junior High School teachers whose qualifications are better? To answer this, this survey was designed to find out find out whether differences exist in Junior High School teachers’ knowledge of integrated science based on their qualifications. In this survey, 57 Junior High School integrated science teachers were sampled from 83 Junior High School integrated science Teachers in the Assin North municipal. The Junior High School integrated science teachers’ knowledge was tested using Junior High School Integrated Science Teachers’ Knowledge Assessment Tool. The scores obtained were analyzed using both descriptive and inferential statistics. The study found that beyond SSSCE/WASSCE level, teachers’ qualification does not bring about significant gains in Junior High School integrated science teachers’ knowledge of integrated science.