The aim of this research to determine the closeness of the relationship between organizational culture, knowledge management and personality with the performance of lecturers. This research use a mixed method with sequential explanatory design. Population in this research is permanent lecturer at Private Academy of Midwifery in City of Jambi, Indonesia amounted to 131 lecturers. The number of samples in this research were 99 lecturers obtained by Slovin formula and regression test was used to examine the correlation between variables. Based on the results of quantitative and qualitative research, this research found: (1) There is a highly significant positive relationship between organizational culture and performance of lecturer with correlation coefficient = 0.61 (p <0.01), and coefficient of determination r = 0.377 or 37.7%; (2) There is a highly significant positive correlation between knowledge management with performance of lecturer with correlation coefficient = 0.479 (p <0,01), and coefficient determination = 0.229 or 22.9%; (3) There is a highly significant positive relationship between lecturer's personality and performance of lecturer with correlation coefficient = 0.454 (p <0,01), and coefficient of determination = 0.206 or 20.6%; (4) There is a highly significant positive relationship between organizational culture, knowledge management and lecturer personality together with lecturer performance with correlation coefficient = 0.594 (p <0,01), and coefficient of determination = 0.353 or 35.3 %. Based on the research findings, the stronger the organizational culture, the more effective the knowledge management and the stronger the personality of the lecturers together will result in the higher level of performance of lecturer.