This study aims to investigate the relationship between transformational leadership, organizational culture and work motivation towards Teacher Performance. The sample selected amounted to 135 people with the criteria of teachers of Public Madrasah in Sarolangun District, Jambi Province. This study uses Mixed Method Sequential Explanatory, which is the first study using quantitative methods than to deepen the research using qualitative methods. This is intended to make the results of the study more in-depth and comprehensive. The results of this study conclude that: (1) Transformational Leadership contributed 24.11%, to Teacher Performance, (2) Organizational Culture contributed 10.1% to Teacher Performance (3) Work Motivation contributed 9.49% through Teacher Performance, (4) Transformational Leadership, Organizational Culture and Work Motivation together contributed 42.25% with Teacher Performance. Other factors are also positively related to the increase in Teacher Performance, which is equal to 57.75%. The results of qualitative research reinforce the results of this quantitative study, and all variables can contribute to improving teacher performance.