Objectives: To assess the bacteriological profile of neonatal sepsis and its antibiotic susceptibility. Methods: A 4 ml paired blood sample was collected in two culture vials at the time of enrolment and 24 hours after enrolment in case of suspected sepsis and sent for bacteriological assessment and antibiotic susceptibility pattern at the Department of Pathology and microbiology. Patients were observed for the outcome in terms of morbidity status, mortality and duration of hospital stay. Results: Out of 180 cases of suspected sepsis, 51 (28.3%) cases were culture positive, Gram positive pathogens were (n=32) (17.8%) and Gram negative pathogens were (n=19)(10.6%).Common isolates were CONS (n=32), S.aureus (n=1), Enterococcus (n=5) & Streptococcus (n=1) in gram positive and K. pneumoniae (n=11), P. aeruginosa (n=3), Acinetobacter (n=1) &E. coli (n=4) in gram negative while contamination was ~1%. Gram positive isolates were highly sensitivity to Tigecycline (93.8%), Nitrofurantoin (69.1%), Teicoplanin (54.5%), Linezolid (53.3%) while Gram negative isolates to Tigecycline (100%), Colistin (81.3%), Meropenem (61.8%). Conclusion: The bacteriological profile of the neonates with sepsis showed a dominance of Gram positive (CONS) as compared to Gram negative isolates (Klebsiella pneumonia). Tigecycline and Colistin had the maximum sensitivity for both Gram positive as well as Gram negative isolates.