Mycobacterium cell envelope is a house of variety of biologically active molecules of different shapes. The proteins associated with cell wall are the key determinants of pathogenesis & immunogenecity.

The whole genome sequence of Mycobacterium tuberculosis, which is 44,03,837 nt in length was downloaded from NCBI site. The ORFs were analyzed by PSORTb v2.0 for the prediction of the subcellular localization of protein. The non-cytoplasmic sequences were used for the further analysis.

The prediction of signal peptide and transmembrane helics was, done by using the PHOBIUS. The identification of the putative virulence factors was carried by searching for homological sequences in the VFDB (Virulence factor database.) Pfam Database was used to find out most potent putative vaccine candidates.

These proteins hold the promise of being potential vaccine candidates for developing an effective vaccine against this disease.