Polythene is the most common plastic & it is the mixture of polymers & ethylene. Polythene play a vital role in packaging of goods, food material, medicine, garbage bags etc. and now a days its degradation is becoming a great threat against the cause of environmental pollution. There are various methods available for the degradation of polythene which are ecofriendly and more acceptable.

The present study deals with the isolation, identification, screening, and degradation of pretreated polythene by microorganism present from soil.

A total of 15 bacteria were recovered from different areas and further screening of polythene degrading microorganism was done by zone of clearance method and out of that 15 bacteria only 3 bacteria showed a positive result and identified to be Staphylococcus sp., Pseudomonas sp., and Bacillus sp. And according to that Staphylococcus sp showed 20% of degradation followed by Pseudomonas sp with 7.5% and Bacillus sp showed a maximum degradation i, e 42.5% in 40 days.

In this way we can conclude that the Bacillus sp. may act as a solution of problem caused by polythene in nature.